Claire Perruisseau-Carrier

Human stem cells & neurosciences:
= Process of human umbilical cord and cord blood
= Isolation of human umbilical cord mesenchymal stem cells (hUC-MSC)
= Expansion of hUC-MSC:
- in classical serum contening culture conditions
- in challenging serum-free culture conditions
= Establishment of a protocol for the commitment of hUC-MSC to neuronal lineages

Development of clinical-friendly protocols for translational and clinical research in stem cells and regenerative medicine.

Modulation of HEK293 cellular pathways for the production of
recombinant proteins in mammalian cells, using miRNAs.
Modelisation of the cellular pathways to be targeted by the miRNA of
choice.
Establishment of a miRNA expression profiling in HEK293 cells.
Establishment of cDNA and miRNA transfection protocols in a 96-well
plate format.
Development and optimization of methods for the evaluation of miRNA
impact on target mRNA expression level.
Optimization of ELISA protocol in order to evaluate the impact of the
transfection of miRNAs on recombinant monoclonal antibody production
level.

Study of the role of methylation in TTF-2 gene expression in SK Hep1
cells.
Bioinformatic characterization of the TTF-2 promoter
Study of differential methylation of TTF-2 promoter CpG sites among cell
lines
Influence of 5-aza-dC treatment on TTF-2 expression in SK Hep1 cell line