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Benjamin PELLETIER

Bagneux

En résumé

Initially, I graduated from ENSTBB in 2005 as a Biotechnological Engineer (Master's degree).


I work at the moment as a Researcher in a French biotechnological company named DBV Technologes.

Recently, I had been working for Bio-Rad Laboratories as a Project Leader in the Immuno Hematology division.
My main tasks led me to coordinate a team of two technicians and to supervise projects to produce monoclonal antibodies targeting red blood cell antigens used in the Luminex (Bioplex) technology for the development of diagnostic tests. This job allowed me to acquire a solid knowledge in Hematology and organization and communicative skills.

Before this experience, I used to work as a Research Engineer in a company specialized in immunotherapy and was in charge of the Research Department.
My responsibilities consisted in discovering and characterizing the effects of immunostimulating proteins (therapeutic antibodies and recombinant vaccines) targeting human activated T cells and dendritic cells in oncology, infectious disease and autoimmune disorder experimental models. In order to study the potency of these molecules, my work led me to practice a broad range of techniques used in the different fields applied to Biology such as molecular biology, cellular biology and biochemistry.
Besides, in order to support my research investigations, I could acquire a proven proficiency in designing in vitro cell based assays by having the responsibility to develop and validate immunological experiments in order to analyse the functional properties of these molecules on the host immune response (cell binding assay, phenotypings, apoptosis assay, cytokine release by Multiplex, cell cytotoxicity and proliferation assays).

Therefore, with these projects, I could gain a real flexibility, autonomy and solid skills in Immunology.

Mes compétences :
Immunology
Biochemistry
Molecular biology
Cellular biology

Entreprises

  • DBV Technologies - Chercheur

    Bagneux 2014 - maintenant Caractérisation des mécanismes immunologiques induits par le traitement de désensibilisation EPIT.
  • Inserm - Ingénieur d'étude

    PARIS 13 2011 - 2014 En charge de mener le projet ERC HYGIENE (European Research Councils) (Pr. Jean François Bach):

    • Etude des mécanismes immunologiques et cellulaires impliqués dans l’allergie, l’asthme et les maladies auto-immunes.

    • Générer des protocoles expérimentaux pour développer des modèles murins de dermatite atopique / asthme.

    • Coordonner et développer des essais cellulaires in-vitro pour induire des réponses immunitaires (Th1/Th2) à partir de traitements thérapeutiques.

    • Déterminer le rôle des différents types cellulaires (cellules dendritiques / lymphocytes T) dans la régulation des maladies immunologiques.
  • Bio-Rad Laboratories - Project Leader

    Marnes La Coquette 2009 - 2011 From June 2009-January 2011 (1 ½ year): Project Leader in Bio-Rad Laboratories (France, Immuno-Hematology Division):

    In charge of:
    - Coordinating projects inside the Monoclonal Antibodies department.
    - Supervising a team of two technicians.

    In charge of managing projects for the development, production and purification of monoclonal (Murine / Human) antibodies targeting human red blood cell antigens for diagnostic tests.

    In charge of designing immunization protocols, screening and characterization assays.

    In charge of transferring Master cell bank files to the Production department.

    In charge of writing laboratory activity report, managing laboratory meetings.
  • IMMUTEP S.A. - Research Engineer

    2005 - 2009 In charge of three projects in the Research department:

    1) Characterization of the inflammatory effects of IMP321 (LAG-3 soluble recombinant protein) clinical grade on Human PBMCs.
    Take part in the publication: A Soluble Form of Lymphocyte Activation Gene-3 (IMP321) Induces Activation of a Large Range of Human Effector Cytotoxic Cells (Chrystelle Brignone, Caroline Grygar, Manon Marcu, Knut Schäkel and Frédéric Triebel)


    2) Project ImmuTune (IMP701 and IMP731): In charge of the development of two types of therapeutic antibodies targeting a human immuno-stimulating protein (LAG-3 / CD223) expressed on T activated / T-reg lymphocytes in oncology, infectious diseases and auto immune disorders:

    - IMP701: Antagonist antibodies designed to increase T cell proliferation against cancer and infectious diseases.

    - IMP731: Cytotoxic antibodies for depleting activated T cells in autoimmune diseases.

    Immutep and GlaxoSmithKline sign licence agreement for IMP731, a novel therapeutic antibody for the treatment of autoimmune diseases: http://www.immutep.com/news/PR23.pdf

    Responsibilities :
    - Immunizations in Balbc strain mice.
    - Hybridoma fusion.
    - Selection (design and validation of high throughput screening protocols).
    - Clonings, MCB and WCB establishment, small scale production.
    - Purification by affinity chromatography.
    - Characterization of the functionalities (in-vitro cell based assays): Cell binding assay,
    immunophenotypings, apoptosis assays, cytokine secretion by Multiplex, cell cytotoxicity and
    proliferation assays, intracellular signalling assay.

    3) Project ImmuCcine: Production, purification and characterization of two recombinant immuno-stimulating vaccines to create therapeutic vaccines against HPV-16 (soluble LAG-3 fused with E7 protein) and HIV-1 (soluble LAG-3 fused protein with Gag and Nef antigens). (IMP361 / IMP362)
  • IMMUTEP S.A. - Internship

    2004 - 2005 From September 2004 to March 2005 (6 months): Internship as an Engineer in Immutep S.A. (France)

    Internship Subject: Development, optimization and validation of the production of IMP361 / IMP362.

    - Selection and clonings of CHO cell lines.
    - Protein expression analysis (biochemical techniques and immunofluorescence by flow cytometry).
    - Production, purification and functionality assays on human dendritic cells.

Formations

  • Ecole Supérieure Des Technologies Des Biomolécules De Bordeaux ESTBB (Bordeaux)

    Bordeaux 2002 - 2005 Biotechnology

Réseau

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