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Julie COULOT

Bois-Colombes

En résumé

Mes compétences :
Tumor
Western Blotting
Oncology
Microscopy
phage display selection
Volunteering
Journals
Genetics
Cell Culture
Affinity Chromatography

Entreprises

  • IBMC - Internship Master 2

    Bois-Colombes 2014 - 2015 Topic: Multiple approach to anti-tumor therapy using lentiviral vectors
    Techniques: Mutagenesis by reconstructive PCR, cloning, DNA purification, protein quantification
    (Bradford), Western Blot, transduction, transfection, mammalian cell culture (human tumor cells adherent and
    in suspension), survival tests (trypan and MTT), development of drug resistant cells, production of viruses
    using embryonic cells (HEK293T), FACS, fluorescent microscopy
  • IGBMC - Internship Master 1

    CHAMBRAY LES TOURS 2014 - 2014 Topic: Study of the interaction between BIN1 and dynamin 2 in myotonic dystrophy I
    Techniques: Analysis of protein interactions (co-immunoprecipitation), protein quantification (Bradford),
    protein purification (affinity chromatography), DNA purification, Western Blot, cloning, transfection,
    production of recombinant proteins using tumor cells (COS-1), fluorescent microscopy
  • Novartis - Summer Trainee

    RUEIL MALMAISON 2013 - 2013 Topic: Construction of plasmids and verification by sequencing
    Techniques: Cloning (PCR, enzymatic digestion, bacterial transformation and DNA purification)
  • Universitatspital Basel - Internship DUT 2

    2011 - 2011 Topic: Comparison of gene expression of Indoleamine-2, 3 dioxygenase in healthy tissue and tumor bladder
    Techniques: RT-qPCR, RNA purification, FACS
    Publication: Mengus Chantal, Schultz-Thater Elke, Coulot Julie, Kastelan Zeljko, Goluza Eleonora, Coric
    Marijana, et al. (2013): MAGE-A10 cancer/testis antigen is highly expressed in high-grade non-muscleinvasive bladder carcinomas. International journal of cancer. 132 (10): 2459-63.

Formations

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