LUDOVIC PECQUEUR

Mission:
Selection of DARPins directed against a biological target using Ribosome Display.
Biochemical characterisation of the selected DARPins.
Cristallogenesis of protein-DARPin complexes.
Structure resolution of tubulin-DARPin complexes

Skills:
Ribosome Display
Molecular biology
Protein production and purification.
Biochemical/Biophysical characterisation using various techniques (Fluorescence, DLS, SEC-MALLS, stopped-flow, cristallography)

Biologie moléculaire
Production de protéines

Mission:
Characterisation of the protein capsid from HIV-1 and its interaction with an inhibitory peptide using NMR.

skills:
Protein expression/purification and isotopic labelling for NMR studies
NMR relaxation data analysis
Residual Dipolar Coupling analysis
3D-structure calculation with NMR data

Master student supervision.
Project management
Python scripting

Thèse CEA:

Study of the Ferric uptake Regulator:
NMR + cristallography + biochemical caracterisation of FUR