Pierre-François ROUX

En résumé

Entreprises

• Institut Pasteur - Postdoctoral Researcher

  Paris 2015 - maintenant Toward a systems view of cellular senescence.
  
  Cell fate decisions entail organized changes in gene expression. A fundamental question
  in biology is how genes and regulatory regions are organized and coordinated for
  transcription regulation in these processes. Recent findings, including those of the
  Bischof team, have demonstrated that alterations in chromatin architecture play a
  decisive role in gene-regulation, but their importance in cell-fate decisions is still unclear.
  To close this important gap in our knowledge, we seek to explore the role of chromatin
  structure changes in cellular senescence, a cell-fate crucial for tumor suppression
  and aging. We hypothesize that active co-regulated genes and their regulatory factors
  coalesce in enhancer-promoter hot spots optimized for efficient and coordinated
  transcriptional control to enforce the senescence phenotype. To prove this hypothesis
  correct, we will combine genome-wide gene expression, epigenome and chromatin
  structure studies in cells undergoing senescence and leverage the expertise of the
  Bischof lab in these domains and my own expertise in high-throughput
  sequencing and transcriptomic data analyses.

• INRA - Doctorant

  Paris 2011 - 2014 Detecting single nucleotide polymorphisms at the genome scale for the genetic dissection of complex traits and the characterization of editomes
  
  Next-generation sequencing technologies (NGS) have progressively revolutionized genetic and transcriptomic research fields since their democratization at the end of the 2000s. While there were mainly used to decipher genomes for many species, major sequencing cost decreases gradually allowed studies focusing on individual genomes and transcriptomes. Studies I have been working on durning my PhD highlight the potential of single nucleotide polymorphisms detection in a high-throughput manner at the genome and transcriptome-wide scales thanks to NGS, for the genetic dissection of complex traits, and for the identification of RNA editing events. The original aspect of our works is lying on the integrative strategy we used to genetically dissect the adiposity, based on several genetic and expressional approaches such as QTL and selective sweep mappings, SNP annotation and prioritization, differential expression and allele-specific expression analyses. Combining those complementary approaches allowed us to shed light on two new candidate genes for the adiposity regulation: JAG2 and PARK2. Focusing on the description of RNA editing events, we report the first studies describing editomes in a non-mammalian vertebrate species - the chicken - and highlight how limited is this phenomenon. We also reveal that environmental and genetic factors such as sex, age, genotype and diet regulate editing levels. Currently, some artifacts related to NGS are still neglected in many studies. Here, we clearly show that ignoring them may deeply impact analyses focusing on rare events such as RNA editing.

Formations

• Université Rennes 1

  Rennes 2009 - 2011 Master

• Ecole Nationale Supérieure Agronomique

  Rennes 2009 - 2011 Ingénieur agronome

• Université Lille 1 Sciences Et Technologies

  Villeneuve D’ascq 2008 - 2009 Master

• Université Lille 1 Sciences Et Technologies

  Villeneuve D'Ascq 2006 - 2008 Licence

• Lycée Albert Châtelet Prépa Bio

  Douai 2004 - 2006

Réseau

• Bastien SADOUL

• Cécile SAUDER

• Delphine GIRAUD

• Guillaume LE MIGNON

• Julie VUILLEMIN

• Karin SIMONIN- LE JEUNE

• Paula ANEB

• Sylvain THIÉBAUT