Steve REYNAUD

En résumé

Entreprises

• CEA Saclay - Intern

  Gif-sur-Yvette 2016 - 2016 Hits confirmation (Venomics Project) : toxins as drug candidates
  Methods:
  Peptide production and purification: reverse-phase HPLC, mass spectrometry (MALDI-TOF, ESI-IT), LC-MS, spectrophotometer
  
  Affinity and selectivity studies: Binding assay using a radioligand, cAMP assay See less

• Institute for Molecular Bioscience, The University of Queensland - Intern

  2015 - 2015 Defining the binding site of Mambalgin on ASIC1a and understanding the molecular basis for the difference in potency against rat and human channel
  Mambalgins are venom peptides that potently inhibit some subtypes of Acid-Sensing Ion Channel (ASIC) and have promising analgesic activity in rodent models of pain
  Techniques and Skills:
  - Recombinant peptide production and purification : Bacterial culture expression, affinity chromatography, HPLC, mass spectrometry MALDI-TOF, ESI)
  - Ion channel mutagenisis, expression and characterisation. (primer design, quick change mutagenesis, in vitro RNA production)
  - Electrophysiological assessment of the inhibitory activity of peptides using two-electrode voltage clamp on Xenopus oocytes
  

• Institut de Pharmacologie Moléculaire et Cellulaire, CNRS - Intern

  2014 - 2014 Pharmacological characterization of ASIC ion channels thanks to animal toxins and marine sponge alkaloids
  
  The activation of Acid Sensing Ion Channels (ASIC) by acidosis and their expression in sensory neurons makes them very interesting novel pain targets. In pursuit of this aim, animal toxins and natural alkaloids could be exellent pharmacological tools.
  
  - Peptide purification : RP-HPLC, cation exchange chromatography, mass spectrometer (MALDI-TOF)
  
  - Toxin activity : Two-electrode voltage clamp on Xenopus oocyte (Roboinject and Roboocyte)

• Centre de Recherche en Neurobiologie et Neurophysiologie de Marseille, CNRS - Intern

  2013 - 2013 Study of inter individual venom variation of Dendroaspis viridis using UHPLC/MALDI-TOF-TOF
  
  - Qualitative and Quantitative study : UHPLC/MALDI-TOF mass spectrometer
  
  - Result analysis : Excel matrix

• Reptilarium du Larzac, Saint-Eulalie de Cernon - Animal keeper

  2012 - 2012 Animals : pythons, boas, monitor lizards, turtles, iguanas, alligator
  
  - Guided tour
  - Animal care
  - Feeding

Formations

• Université Paris-Saclay

  Saclay 2016 - maintenant Thèse
  
  Animal toxins as innovative ligands for G Protein-coupled Receptor (GPCR)
  Aims :
  Following High troughput screening, production and characterization of the first animal toxins active on melanocortin 4 receptor (a GPCR expressed in hypothalamus)
  Optimization of the best hit as a potential lead in eating disorders: easy and efficient production, affinity and selectivity improvement
  
  Methods :
  Pept

• Polytech'Nice-Sophia

  Sophia Antipolis 2013 - maintenant

• Université Toulouse 3 Paul Sabatier

  Toulouse 2011 - 2013 Licence générale Biologie et Environnement parcours Biologie des Organismes, des Populations et des Ecosystèmes
  
  Equivalent to a three-year university degree in Ecology and Evolutionary Biology

• Université Clermont 1 Auvergne

  Clermont Ferrand 2009 - 2011 DUT Génie Biologique option Agronomie
  
  Equivalent to a two-year university degree in technology in Biological Engineering

Réseau

• Adrien COUSY

• Alexandre MOHAMED-ALI

• Cecile BIGAND

• Christine RISSO

• Congcong ZHAO

• Julien PECHER

• Landry GAYET

• Marina GROUSSARD

• Sandra ROGER