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François LE FLOCH

WAVRE

En résumé

Mes compétences :
Cell culture
Chromatography
Culture
DNA
Downstream
Filtration
Process
Process Development
Purification
Scale up
Upstream

Entreprises

  • ThromboGenics - Purification Expert & Team Leader

    2009 - maintenant - Purification and analysis of monoclonal antibodies (MAbs) for pre-clinical use (1-10 g scale)
    - Process development for the purification of recombinant proteins (IEX, IMAC, affinity...)
    - Preclinical production Team leader: supervison of USP & DSP activities (& molecular biology)

  • Vivalis - Downstream Process Development, Head

    Roussay, 2007 - 2009 Process development for the purification of viral vaccines (MVA) and monoclonal antibodies MAbs (2-20 L scale)
    - Development of purification processes: cell lysis, clarification, tangential flow filtration (TFF flat sheets and hollow fibers), ion-exchange on membrane, affinity chromatography, gel filtration (SEC), Benzonase digestion…
    - Large contribution to GMP transfer (up-scaling to 100 L, equipment design, FAT)
    - Study of the potential contaminants: host-cell proteins, DNA, virus clearance
    - Involvement in project management with industrial partners
    - Coaching of 2 engineers

  • Cayla - InvivoGen - Production of recombinant proteins, Head (cell culture & purification)

    Villefranche-de-Rouergue 2005 - 2007 Process development for the production of recombinant cytokines, antibodies (PAbs, IgA), receptors…
    - Development of purification processes: ion exchange (IEX), affinity and HIC chromatography, SEC, tangential ultra-filtration…
    - Stability optimization during lyophilization (addition of stabilizers)
    - Development of mammalian cell culture processes: transient expression by HEK 293 cells, cell adaptation to serum-free culture (suspension), transfection optimization
    - Coaching of 2 technicians

  • MolMed - Pilot plant scientist for the purification of viral vectors & recombinant proteins

    2002 - 2005 Development of purification and culture processes for the production of viral vectors and recombinant proteins (cytokines and MAbs), set-up of analytical methods
    - Development of processes for the purification of retroviral particles and recombinant proteins: TFF, SEC, HIC, IEX, affinity, RP-chromatography, precipitation, DNase treatment…
    - Up-scaling to 20 L for TFF process (Pilot plant)

Formations

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