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ULB- laboratoire du Métabolisme de l'ARN (D. Lafontaine)
- Post-doc, chargé de recherches FNRS
2009 - maintenant
Identification et caractérisation de facteurs protéiques requis dans la biogenèse du ribosome chez l'homme
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INSERM U851, équipe I-MAP (V. Lotteau, C. Rabourdin-Combe)
- Post-doc
2007 - 2009
Cartographie et caractérisation des interactions physiques entre protéines de la polymérase de la grippe et des proteins cellulaires. projet de recherche européen “FluPol” (n° FP6-2005-SSP-5B) : “ Host specific variants of the Influenza virus replication machinery ”.
Publications scientifiques:
- "Generation and comprehensive analysis of an influenza virus polymerase cellular interaction network."
Tafforeau L, Chantier T, Pradezynski F, Pellet J, Mangeot PE, Vidalain PO, Andre P, Rabourdin-Combe C, Lotteau V. J Virol. 2011 Dec;85(24):13010-8.
Accès: http://jvi.asm.org/content/85/24/13010.long
http://flupol.lyon.inserm.fr/ (réseau d'interactions)
- "Virus-Human Cell interactomes."
Tafforeau L, Rabourdin-Combe C, Lotteau V. Two-hybrid technologies- methods and protocols. Methods Mol. Biol. 2012;812;103-120.
Accès: http://www.springerlink.com/content/j056l56610603617/#section=1013410&page=1
- "The Interactomes of Influenza Virus NS1 and NS2 Proteins Identify New Host Factors and Provide Insights for ADAR1 Playing a Supportive Role in Virus Replication."
de Chassey B, Aublin-Gex A, Ruggieri A, Meyniel-Schicklin L, Pradezynski F, Davoust N, Chantier T, Tafforeau L, Mangeot PE, Ciancia C, Perrin-Cocon L, Bartenschlager R, Andre P, Lotteau V. Plos Path 2013, 9 (7): e1003440.
Accès: http://www.plospathogens.org/article/info%3Adoi%2F10.1371%2Fjournal.ppat.1003440
Collaborations :
- "Comparative analysis of virus-host interactomes with a mammalian high-throughput protein complementation assay based on Gaussia princeps luciferase."
Neveu G, Cassonnet P, Vidalain PO, Rolloy C, Mendoza J, Jones L, Tangy F, Muller M, Demeret C, Tafforeau L, et al. Methods 2012 Dec;58(4):349-59.
Accès: http://www.sciencedirect.com/science/article/pii/S1046202312001855
- "Mapping of Chikungunya Virus Interactions with Host Proteins Identified nsP2 as a Highly Connected Viral Component."
Bouraï M, Lucas-Hourani M, Gad HH, Drosten C, Jacob Y, Tafforeau L, Cassonnet P, Jones LM, Judith D, Couderc T, Lecuit M, André P, Kümmerer BM, Lotteau V, Desprès P, Tangy F, Vidalain PO. J VIrol. J Virol. 2012 Mar;86(6):3121-34. Epub 2012 Jan 18.
Accès: http://jvi.asm.org/content/86/6/3121.long
-"Epstein-Barr virus protein EB2 stimulates cytoplasmic mRNA accumulation by counteracting the deleterious effects of SRp20 on viral mRNAs."
Juillard F, Bazot Q, Mure F, Tafforeau L, Macri C, Rabourdin-Combe C, Lotteau V, Manet E, Gruffat H. Nucleic Acids Res., 2012, epub ahead of print
Link:http://nar.oxfordjournals.org/content/early/2012/04/13/nar.gks319.long
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INSERM U851, équipe I-MAP (V. Lotteau, C. Rabourdin-Combe)
- Post-doc
2005 - 2006
- Mise au point et développement d’une plate-forme de protéomique fonctionnelle (interactomique) utilisant la technique du crible-hybride en levure et le clonage par recombinaison in vitro (gateway);
- Cartographie et caractérisation des interactions entre protéines du virus de l’hépatite C et des protéines cellulaires.
Publications scientifiques:
- "ViralORFeome: an integrated database to generate a versatile collection of viral ORFs."
Pellet J.*, Tafforeau L.*, Lucas-Hourani M.*, Navratil V., Meyniel L., Achaz G., Guironnet-Paquet A., Aublin-Gex A., Caignard G., Cassonnet P., Chaboud A., Chantier T., Deloire A., Demeret C., Le Breton M., Neveu G., Jacotot L., Vaglio P., Delmotte S., Gautier C., Combet C., Deleage G., Favre M., Tangy F., Jacob Y., Andre P., Lotteau V., Rabourdin-Combe C. and Vidalain PO. Nucleic Acids Res. 2010 Jan;38(Database issue):D371-8. Epub 2009 Dec 8.
Accès: http://nar.oxfordjournals.org/content/38/suppl_1/D371.long
http://www.viralorfeome.com (base de données)
- "pISTil: a pipeline for yeast two-hybrid Interaction Sequence Tags identification and analysis."
J. Pellet, L. Meyniel, PO. Vidalain, B. de Chassey, L. Tafforeau, V. Lotteau, C. Rabourdin-Combe and V. Navratil. BMC Research Notes 2009, 2:220.
Accès: http://www.biomedcentral.com/1756-0500/2/220
- "The HCV protein infection network."
B. de Chassey*, V. Navratil*, L. Tafforeau*, M. S. Hiet, A. Aublin-Gex, S. Agaugué, G. Meiffren, F. Pradezynski, B.F. Faria, T. Chantier, M. Le Breton, J. Pellet, N. Davoust, P. E. Mangeot, A. Chaboud, F. Penin, Y. Jacob, P.O. Vidalain, M. Vidal, P. André, C. Rabourdin-Combe and V. Lotteau. Mol Syst Biol. 2008;4:230. Epub 2008 Nov 4.
Accès: http://www.nature.com/msb/journal/v4/n1/full/msb200866.html
*: contribution égale des auteurs
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FUNDP, Laboratoire de Génétique Moléculaire (J. Vandenhaute)
- Doctorant
2000 - 2005
Approche génétique et biochimique du rôle de protéines à F-box chez Schizosaccharomyces pombe.
Publications:
- "Recruitment of P-TEFb (CDK9-Pch1) to chromatin by the cap-methyl transferase Pcm1 in fission yeast."
A. Guiguen, J. Soutourina, M. Dewez, L. Tafforeau, M. Dieu, M. Raes, J. Vandenhaute, M. Werner and D. Hermand. EMBO J., (2007), 26, 1552-1559.
Accès: http://www.nature.com/emboj/journal/v26/n6/full/7601627a.html
- "Repression of ergosterol level during oxidative stress by fission yeast F-box protein Pof14 independently of SCF."
L. Tafforeau, S. Le Blastier, S. Bamps, M. Dewez, J. Vandenhaute and D. Hermand. EMBO J., (2006), 25, 4547-4556.
Accès: http://www.nature.com/emboj/journal/v25/n19/full/7601329a.html
- "Additional vectors for PCR-based gene tagging in Saccharomyces cerevisiae and Schizosaccharomyces pombe using nourseothricin resistance."
B. Van Driessche*, L. Tafforeau*, P. Hentges, A. M. Carr and J. Vandenhaute. Yeast, (2005), 22, 1061–1068.
Accès: http://onlinelibrary.wiley.com/doi/10.1002/yea.1293/abstract;jsessionid=A8B140C2ECF5C74330E43A12071C8A09.d01t01
- "Three novel antibiotic marker cassettes for gene disruption and marker switching in Schizosaccharomyces pombe."
P. Hentges, B. Van Driessche*, L. Tafforeau*, J. Vandenhaute and A. Carr. Yeast, (2005), 22, 1013-1019.
Accès: http://onlinelibrary.wiley.com/doi/10.1002/yea.1291/abstract;jsessionid=798A4A5C167EA93C34046BAF0B1AA6FC.d01t03
- "Mcs2 and a novel CAK subunit Pmh1 associate with Skp1 in fission yeast."
S. Bamps*, T. Westerling*, A. Pihlak, L. Tafforeau, J. Vandenhaute, T.P. Makela and D. Hermand. Biochem. Biophys. Res. Commun., (2004), 325, 1424-1432.
Accès: http://www.sciencedirect.com/science/article/pii/S0006291X04025252
- "Skp1 and the F-box protein Pof6 are essential for cell separation in Fission Yeast."
D. Hermand*, S. Bamps*, L. Tafforeau, J. Vandenhaute and T.P. Makela. J. Biol. Chem., (2003), 278, 9671-9677.
Accès: http://www.jbc.org/content/278/11/9671.long
*: contribution égale des auteurs
