Loïc DAYON

En résumé

Entreprises

• Nestlé Institute of Health Sciences - Proteomics/Metabolomics Scientist

  Marne La Vallée Cedex 2 2011 - maintenant

• Proteome Sciences plc - Senior Research Scientist

  2010 - 2011 Development of multiplexed methods for selected reaction monitoring (SRM) mass spectrometry-based quantification of protein biomarkers using triple quadrupole (QqQ) mass spectrometers.

• Université de Genève - Post-doc/Research Associate

  Genève 4 2007 - 2009 • Mass spectrometry and analytical development: Relative quantification of proteins using 6-plex isobaric tagging. Hyphenation of isobaric tagging and off-gel electrophoresis for efficient quantitative analysis of protein. Absolute quantitation of protein markers with reporter calibration curves. Optimization of quantitation by isobaric tagging using a linear ion trap-orbitrap hybrid mass spectrometer.
  • Proteomics and biomarker discovery: Discovery of brain damage markers in cerebrospinal fluid. Quantitative analysis of human brain microdialysis samples of stroke patients.
  
  Keywords: ESI and MALDI mass spectrometry (ion trap, TOF/TOF, Q-TOF, triple quadrupoles, orbitrap). Liquid chromatographic separation (normal phase, RP, SCX, immuno-affinity). IEF, OFFGEL electrophoresis. LC MS/MS. Protein indentification and quantitation. Tandem mass tags. iTRAQ.

• EPFL - PhD Student and Post-doc

  2002 - 2006 • Mass spectrometry and electrochemistry: Electrochemical tagging during electrospray ionization for counting of cysteines in peptides/proteins and enhanced protein identification by mass spectrometry.
  • Liquid chromatography: Combined fractional diagonal chromatographic (COFRADIC) selection of cysteinyl peptides with benzoquinone tags.
  • Development of electrospray ionization sources: mutli-electrospray chip for screening of compounds, integration of electrode arrays in polyethylene terephthalate microchip, mixer implementation in microspray interface.
  • Chemical kinetics: Numerical study of consecutive reaction whitin microchannels. Evaluation of reaction kinetics for electrochemical tagging with benzoquinone compounds.

• Actelion Pharmaceuticals - Trainee

  2002 - 2002 Synthesis of protease inhibitors (optimization of structurally simple lead compounds, investigation on the Suzuki reaction).

• Roche Vitamines France - Engineer Trainee

  2001 - 2001 Optimization of the cantaxanthine and riboflavine-5’-phosphate syntheses (redefinition of initial material amount on a pilot plant and application to the full-scale industrial production).

• F. Hoffmann-La Roche - Technician Trainee

  2000 - 2000 Quality control and insurance of pharmaceutical products using especially high performance liquid chromatography and UV analyses, working with good manufacturing practice (GMP).

Formations

• Ecole Polytechnique Fédérale De Lausanne EPFL (Lausanne)

  Lausanne 2002 - 2006 Chimie bioanalytique
  
  Doctorant
  
  Thesis title "Thiol-targeted microspray mass spectrometry of peptides and proteins through on-line EC-tagging"

• Ecole Nationale Supérieure De Chimie De Mulhouse

  1998 - 2002 Chimie organique et bioorganique

Réseau

• Christine HOOGLAND

• Corinne PEYRON - LE ROUSSEAU

• Jessica BERNARD

• Lionel TSCHOPP

• Michel REYMOND

• Olivier CASSE

• Pierre LLOPIZ

• Zineb ABDELLAOUI MAANE