Mes compétences :
Recherche
HPLC
Biochimie
Enzymologie
Biologie moléculaire
Entreprises
Glycom A/S
- Project Manager
2011 - maintenant- Project objectives: Development of new chemo-enzymatic routes to synthesize demanding oligosaccharides including human milk oligosaccharides.
- Set-up of the biocatalysis activities at Glycom A/S
- Initiate/ coordinate R&D programs with external partners to promote development and growth.
- Design, perform and report experimental investigations for assigned projects.
- Safety representative for the Biocatalysis activities
MIT (Massachusetts Institute of Technology)
- Post-doc
2009 - 2011- Project objectives: Prediction of protein-protein interaction sites
Department of Chemical Engineering, Prof. B. Trout Group, MIT. (Cambridge, MA, United States)
In collaboration with Novartis (Basel, Switzerland)
- Contributions: Development of the experimental strategy/ Protein production and purification/ Protein binding assays (TR-FRET assays, cell-based assays) / Stuctural characterization of proteins (MS, CD, SEC-HPLC)
INSA Toulouse (Labo des Systèmes Biologiques et des Bioprocédés)
- PhD/Post-Doc
2004 - 2009- Project objectives: Design of α-transglucosidases of controlled specificity for programmed chemo- enzymatic synthesis of antigenic oligosaccharides.
Department of Biocatalysis, Prof P. Monsan Group. (INSA, Toulouse/France).
Advisor : P. Monsan – Co-Advisors : M. Remaud-Siméon and I. André
In collaboration with Pasteur Institute (Paris, France –Resp : L. Mulard) and CERMAV (Grenoble/France - Resp : H. Driguez).
ANR (Agence Nationale de la Recherche) funded project (2005-2008).
- Contributions: Choice of a semi-rational engineering approach / Identification of key positions relied on docking studies / Construction of enzyme librairies/ Development of enzyme screening protocols on liquid&solid media / Production, purification and biochemical characterization of the most improved variants / Preparative synthesis, purification and stuctural characterization (HPLC, HPAEC, NMR and MS) of target products.
- Achievements: Validation of a new concept (Exploitation of enzyme engineering technologies to create designed glycoenzymes entering into a programmed chemoenzymatic pathway).
